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KMID : 0380020040190030174
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 3 p.174 ~ p.177
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Storage of Pollen Biofactory for in vitro Growth and rProtein Synthesis
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Park Hee-Sung
Ko Jae-Chul
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Abstract
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A method of collection and long-term storage of viable lily (Lilium longiflorum) pollen grains were developed for their in vitro growth and transformation in consistency. Petroleum ether, n-heptane, cyclohexane and benzene, as pollen collection medium, were determined less toxic to pollen growth in vitro than others tested. Pollen grains, however, lost their growth activity if stored in these solvents more than a week, So, a serial performance, that is, pollen grain collection in these solvents, air-drying and immediate transfer to low temperature condition was determined desirable for keeping the viability much longer. Pollen grains from this storage showed a successful transformation in vitro with a cDNA encoding tissue plasminogen activator (TPA) protein using Agrobacterium via vacuum infiltration according to western blotting analysis.
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KEYWORD
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Lily pollen, storage, in vitro growth, TPA transformation, western blotting
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